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The Hepatocyte Review

Author: M. N. Berry

Publisher: Springer Science & Business Media

ISBN: 9789401733458

Category: Science

Page: 606

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It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.
The Hepatocyte Review
Language: en
Pages: 606
Authors: M. N. Berry, Anthony M. Edwards
Categories: Science
Type: BOOK - Published: 2013-06-29 - Publisher: Springer Science & Business Media

It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most
Culture of Epithelial Cells
Language: en
Pages: 461
Authors: R. Ian Freshney, Mary G. Freshney
Categories: Medical
Type: BOOK - Published: 2004-04-07 - Publisher: John Wiley & Sons

"...a wonderful compendium of current in vitro approaches that willbe a useful resource to those just starting to work with anepithelial cell system as well as those that have been working withthem for years and years." —Pharmaceutical Research This completely revised and expanded new edition providesdetailed descriptions of fundamental and
Bioreactors for Tissue Engineering
Language: en
Pages: 375
Authors: Julian Chaudhuri, Mohamed Al-Rubeai
Categories: Medical
Type: BOOK - Published: 2005-10-18 - Publisher: Springer Science & Business Media

Developments in tissue engineering for human medicine are increasing rapidly. Advances in stem cell biology, biomaterials science and scaffold design underpin this emerging science. An equally important facet of this field is the rational design and operation of bioreactors to control the nascent tissue growth. For the first time in
Hepatocytes
Language: en
Pages: 553
Authors: Patrick Maurel
Categories: Medical
Type: BOOK - Published: 2016-08-23 - Publisher: Humana

Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte
Autophagy
Language: en
Pages: 327
Authors: Daniel Klionsky
Categories: Science
Type: BOOK - Published: 2003-12-15 - Publisher: CRC Press

Starting in the early 1970s, a type of programmed cell death called apoptosis began to receive attention. Over the next three decades, research in this area continued at an accelerated rate. In the early 1990s, a second type of programmed cell death, autophagy, came into focus. Autophagy has been studied